PEPperCHIP® Peptide Microarray

PEPperPRINT’s proprietary peptide laser printing technology, the high-density PEPperCHIP® Peptide Microarrays provides a platform for :
 Antigen and Epitope Discovery
 Epitope Mapping
 Epitope Substitution Scans
 Immune Monitoring
 Serum Biomarker Discovery
 Peptide Target Binder Development

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  • Introduction

    PEPperCHIP® Peptide Microarray analysis is straightforward and can be done in your own lab or by PEPperPRINT experts within a PEPperMAP® Service Offer. Further information about lab requirements for peptide microarray analysis can be found here.

    From peptide array design to production and quality control, it takes about 3 weeks.

     Highest spot densities and numbers of peptides per chip
     Widely unlimited peptide content flexibility
     Adjustment of peptide microarray layouts according to your specifications
     Cost-effective solutions without excessive and expensive peptide pre-synthesis
     Multiple peptide array copies per chip for one-by-one assays
     Outstanding signal-to-noise ratios due to custom slide coatings
     Straighforward handling and analysis
     Comprehensive support before, during and after purchase


    Specifications :

    From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.

    Peptide Synthesis: On-chip using Fmoc chemistry and routine double coupling steps
    Microarray Format: Standard slide format (3” x 1”, 75.4 mm x 25.0 mm x 1.0 mm)
    Number of Peptides: From 130 to 5,500 peptides printed in duplicate
    Arrays Copies per Chip: From single arrays to 16 arrays per chip depending on the number of peptides
    Array Content: Custom protein-derived overlapping peptides, peptide collections, substitution scans etc.
    Available Amino Acids: L- and D-amino acids, citrulline and other amino acids on request
    Peptide Length: Routine synthesis of up to 15mer peptides, longer peptides on request
    Control Peptides: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) epitopes, other controls on request

    Input Data & Sample Material :

    10-20 µl serum/plasma or 10-20 µg purified antibody
    Protein sequences (e.g. FASTA format or UniProt ID) or peptide collections (Excel, Word or text files)

    Further Information :

    Application Notes
    Webinars and Movies