Media & Reagents

Cell culture media: An overview

In the field of life sciences, one of the most important methodologies is cell culture. The removal of cells, tissues, or organs from an animal or plant and the subsequent implantation of those cells, tissues, or organs in an artificial environment that is favorable to their survival and/or growth is what is meant by the phrase "cell culture." The fundamental environmental needs for optimal cell development are controlled temperature, a substrate for cell attachment, an adequate growth medium, and an incubator that maintains the optimum pH and osmolality. Cells must have these conditions in order to grow to their full potential.

The selection of an adequate growth medium for in vitro cultivation is the stage in cell culture that is both the most critical and the most vital. A growth medium, also known as culture media, is a liquid or gel formulated to encourage organisms' development on a microscopic, cellular, or plant-like scale. The medium used for cultivating cells often contains an adequate supply of energy and substances that control the cell cycle. The main components of a culture media include amino acids, vitamins, inorganic salts, glucose, and serum. The serum is added to the medium because it acts as a source of growth factors, hormones, and attachment factors. In addition to providing nutrients, the medium also contributes to the upkeep of pH and osmolality levels.

Types of mediums used in cell culture

Natural media

Artificial media