Biotium Tech Tip
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Many researchers are unaware that photoconversion of DAPI and Hoechst can cause nuclear stains to fluoresce in the FITC and Cy®3 channels.
Learn about the different ways to avoid DAPI photoconversion.
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Photoconversion of DAPI to green and red fluorescence after exposure to UV light. Fixed and permeabilized HeLa cells stained with DAPI were imaged by confocal microscopy before or after exposure to mercury lamp excitation through the DAPI filter cube for 3 minutes.