UltraMarathonRT® (uMRT) Direct RNA-Seq Kit

Unlock direct insight into RNA biology with RNAConnect’s UltraMarathonRT® (uMRT) Direct RNA-Seq Kit, the first complete, ready-to-use solution for preparing native RNA libraries for Oxford Nanopore Technologies® (SQK-RNA004) sequencing.

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  • Introduction

    Powered by helicase-driven processivity, uMRT brings greater coverage and sensitivity to direct RNA sequencing workflows, revealing longer transcripts, modification patterns, and transcript structures that other RTs simply miss.

    Why Choose the uMRT Direct Kit?

    • Higher CPMs & Longer Read Lengths
    • This kit delivers >10% higher mapped reads from ≥ 2 kb RNAs. uMRT works at 30°C, which preserves the integrity of longer transcripts.

    • Generate Higher cDNA Yields from your Sample
    • uMRT efficiently converts even low-abundance, structured transcripts, increasing long-read cDNA yields > 25% and improving transcript-level quantification.

    • Optimized for Native RNA
    • uMRT’s high processivity and gentle 30 °C reaction temperature preserves full length, RNA integrity and base modifications— making it ideal for studying epitranscriptomic marks, RNA dynamics, and full-length isoforms.

    • All-in-One Simplicity
    • Eliminate the need to source multiple enzymes and reagents from different vendors. Our 6 & 48 reaction kits and standardized, validated workflow mean you no longer have to order multiple reagents from different vendors in quantities that may not match your experimental requirements.

    Description

    See the data: longer reads, more novel transcripts

    Compared to the existing ONTR protocol, the uMRT Direct RNA-Seq Kit shows:

    • 5–10% longer read lengths (with big gains >2 kb)
    • 25–50% higher cDNA yield
    • 1–2% more total genes and 15–20% more novel transcripts (NIC/NNIC)
    • 25–30% more intron retention events

    Comparison: ONT Standard vs uMRT Direct





    Kit Components:

    • UltraMarathonRT® (20 U/μL)
    • 2x RT Reaction Buffer
    • dNTP mix (10 mM each)
    • RNA Conditioner
    • 5x Ligation Buffer Q
    • DNA Ligase (2,000,000 U/mL)
    • RNase Inhibitor (40 U/uL)
    • Quenching Buffer
    • Nuclease-free Water

    Catalog# R1008S (6 rxns), R1008M (48 rxns)

    Click here for the product brochure.

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