UltraMarathonRT® Two- Step RT-PCR Kit

The UltraMarathonRT® Two-Step RT- PCR Kit offers faithful RNA-to-cDNA conversion followed by robust amplification, regardless of the RNA template’s length, secondary structure, or GC content.

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Introduction

A High-Performance Solution for Sensitive and Precise RNA Analysis

The UltraMarathonRT® Two-Step RT-PCR Kit is a next-generation tool designed for researchers seeking unmatched sensitivity, high-fidelity, and efficiency when analyzing RNA. Whether you are studying complex, long, or structured RNA targets, these kits deliver high-fidelity results with exceptional performance.

Description

Key Features

Unparalleled Sensitivity: Requires as little as 0.1 pg of input RNA to amplify RNAs >10 kb, enabling detection of even low-abundance targets.

Processive Enzyme: Powered by UltraMarathonRT®, an ultra-processive reverse transcriptase with intrinsic helicase activity that actively unwinds RNA secondary and tertiary structures without heat, producing full-length cDNA with exceptional fidelity.
Long-Read Capability: Efficiently amplifies cDNA products up to 30 kb using a highly processive, high-fidelity DNA polymerase.

Versatile Applications: Ideal for experiments requiring accurate gene detection, from short transcripts to tens of thousands of nucleotides in the areas of gene expression analysis, full-length isoform analysis, full-length virus genome analysis, molecular cloning, long or structured RNA analysis with low input.