SiR700-actin kit

SiR700-actin is a far-red, fluorogenic, cell permeable and highly specific probe for F-actin
Kit contains 35 nmol SiR700-actin and 1 umol verapamil

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  • Introduction

    SiR700-actin is based on the F-actin binding natural product jasplakinolide. SiR700-actin is fluorogenic, cell permeable and highly specific for F-actin. Sir700-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR700-actin is compatible with GFP and/or m-cherry fluorescent proteins. SiR700-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 35 – 150 staining experiments.*

    In combination with standard SiR-probes, SiR700-actin allows dual colour imaging experiments in live cells. For more information please visit www.spirochrome.com/dualcolour.

    *Based on the following conditions: 0.5 – 1 ml staining solution / staining experiments with 0.5 – 1 uM probe concentration. The number of staining experiments can be further increased by reducing volume or probe concentration.

    Description

    Optical Properties

    λabs 682 nm
    εmax 1.0·105 mol-1·cm-1
    λfl 716 nm
    Recommended STED depletion line: 810 nm

    Downloads :

    SiR700-actin kit datasheet
    Verapamil usage guidelines
    SiR700-actin SDS
    Ex/Em spectra (excel format)

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