Pump System

A Pump System for Long-Term Cell Culture Under Flow to Mimic Physiologic Conditions

• Establish in vivo-like conditions for the cultivation of cells under flow (e.g., endothelial cells for the simulation of blood vessels)
• Simulate defined laminar flow types (unidirectional, pulsatile, and oscillatory flow) occurring in different tissues and settings
• Compatible with µ-Slides with Luer adapters, to be used with all cell culture incubators, all inverted microscopes, and ibidi Stage Top Incubators

System Includes:

• ibidi Pump
• Fluidic Unit
• Notebook with PumpControl Software
• Perfusion Set of Your Choice

Applications

Many cell types experience moving fluids, such as vascular and lymphatic endothelial cells or kidney and lung epithelial cells. This liquid flow causes shear stress, a mechanical force that significantly impacts the cells’ physiological behavior, structural properties, and function. Cultivating cells under flow in vitro enables studying endothelial and epithelial cells in a more physiological, in vivo-like environment.

• Long-term cell culture under flow with defined shear stress values (e.g., endothelium, kidney, or biofilm).
• Simulation of defined continuous and pulsatile laminar flow, and oscillatory flow with defined shear stress to study cells in a more physiological environment.
• Rolling and adhesion assays using suspended cells (e.g., T cells) on substrates.
• Transmigration and invasion studies.
• Perfusion of single cells, spheroids, and organoids in 2D and 3D for optimal nutrition.
• Live cell imaging and immunofluorescence assays for analyzing shear stress response.

MV 20: The ibidi Pump System