Milo™ is the world’s first Single-Cell Western platform. He measures protein expression in thousands of single cells in a single run so you can profile heterogeneity in your samples. Just load your cell suspension and the scWest chip captures ~1,000 single-cells. Milo then does a fast, 1 minute SDS-PAGE separation on each single-cell lysate on-chip. Then just probe with your favorite conventional Western antibodies to measure up to 4 proteins per cell simultaneously. Use Single-Cell Westerns to unlock the single-cell proteome and measure more of the proteome than is possible with any other single-cell technique.
Milo measures protein expression in ~1,000 single-cells in one 4 hour experiment so you can quantify expression heterogeneity of your target and the percentage of cells in your sample that are target positive.
Requirements & compatibility
|Sample type||Suspension containing >10,000 cells|
|Cell diameter||7-25 µm|
|Cell type||Mammalian cells; globular in suspension and unfixed|
|Antibody requirement||Standard, commercial unlabeled primaries and fluorescent secondaries|
|Other equipment needed||Open-format fluorescence microarray scanner capable of 10 µm resolution|
Performance & specifications
|Typical cell dilutions||Yield capture and analysis of 1,000-2,000 cells per scWest chip|
|Molecular weight (MW) range||15-175 kDa|
|MW resolution||10% differences in distinct spectral channels, as low as 30% differences in same spectral channel|
|Typical target multiplexing||Up to four proteins per cell by spectral and size-based multiplexing|
|Workflow time||4-6 hours|