Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Just load your samples and reagents into the microplate and Jess separates your proteins by size, and precisely manages antibody additions, incubations, washes and even the detection steps. Come back to fully analyzed results in 3 hours. Go further with multiplexing—her fluorescent detection gets all the information you need in one shot. Jess, she’s like Western blot meets ELISA in one.
Jess gives you four different ways to analyze proteins.
Fluorescent detection : Why bother stripping and reprobing? Maximize your time and sample, and get the information you need in one shot, with multiplexing.
Chemiluminescent detection : Working with low abundance targets or precious samples? Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample.
Protein normalization : Jess gives you an easy way to see if your samples contain a consistent protein load—just load her in-capillary protein normalization reagent into her assay plate and she’ll take care of the rest. Her proprietary fluorescent reagent measures proteins immobilized in the same capillary as your immunoassay. The result? You can quickly see if your samples contain a consistent protein load, identify experimental setup and user errors, effectively normalize expression of your target protein to get accurate and consistent data, giving you the confidence you need in your results. Best of all, Jess’s fluorescent detection capabilities enable two-color protein detection for multiplexing on top of protein normalization.
Blot imaging : Still doing traditional Westerns? Snap! Get the picture with Jess’s in-built blot imaging system.Simple Western immunoassays take place in a capillary. Your sample, separation matrix, stacking matrix, antibodies and reagents are loaded automatically from a specially designed plate. Jess begins by aspirating the separation matrix and then the stacking matrix into each capillary.Next your sample is loaded, and capillaries are lowered to make contact with running buffer. Voltage is applied to enable separation by molecular weight. Once the separation is complete, UV light immobilizes the proteins to the capillary wall. With proteins now immobilized and the matrix cleared of the capillary, Jess starts the immunoprobing process, first with incubation with the primary antibody, followed by a secondary HRP conjugate, and finally chemiluminescent substrate. The chemiluminescent reaction is recorded by a CCD camera in a series of images over time.In just 3 hours you’ll have quantitative, size-based data ready for analysis.Her fluorescent detection capabilities enable two-color protein detection for multiplexing. During the immunoprobing process samples are incubated with the primary antibody, followed by infrared or near-infrared fluorescent secondary-tagged antibodies. Excitation of the fluorphores releases photons and the emission spectra is detected by wavelength sensors and recorded by a CCD camera in a series of images over time. In just 3 hours you’ll have multiplexed, quantitative, size-based data ready for analysis.
|Sample Required||0.3-1.2 µg||0.6-1.2 µg||2-4 µg||0.6-4 µg|
|Volume Required||3 µL/well|
|Size Range||Molecular weight (MW) ladder ranges from 2-440 kDa|
|Resolution (± percent difference in MW)||± 15-20% for MW <20 kDA
± 10% for MW >20 kDa
|Dynamic Range||2-3 logs||3-4 logs||3-4 logs||2-3 logs|
|Sensitivity||ng||Low pg||High pg||ng|
|Capillary||5 cm, 100 µm, 400 nL|
|un time||<3 hours||<4 hours with immunoassay|
|Samples per run||13 or 25|
|Dimensions (closed)||0.36 m H X 0.3 m W X 0.57 m D|
|Dimensions (open)||0.36 m H x 0.53 m W x 0.57 m D|
|Power||US/CAN 120 V AC, 60 hz, 4.2 amps
Europe 240 AC, 50 Hz, 2.1 amps
Japan 100 AC, 50/60 Hz, 5.0 amps
|Operating temperature||18-24 °C|
|Operating humidity||20-60% relative, non-condensing|