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ibidi Online Course: Successful Immunofluorescence Image Acquisition and Quantification

Immunofluorescence is a powerful method used to visualize structures and molecules of interest within cells and tissues. However, researchers are often challenged when trying to obtain and process sharp and brilliant images. For successful immunofluorescence images, each step—including cell preparation, staining, and image acquisition—needs to be optimized.

This new ibidi online course is an extension of the previous webinar, How to Set Up a Successful Immunofluorescence Experiment, and is once again hosted by our invited speaker Shuntaro Yamada. During his first webinar, Yamada shared his practical tips and tricks for immunofluorescence staining as he guided us through each step of a standard immunofluorescence protocol. This follow-up course expands on that topic with an in-depth description of how to perform successful image acquisition and quantification.

After completing the course, you will have advanced knowledge and a better understanding of the necessary tools required to process beautiful immunofluorescence stainings.

No sterile working benches or cells are required to participate. Attend the course from the comfort of your office or home.

Course dates (Choose ONE) :
1. March 7, 2023: 11pm–1.30am SGT; OR
2. March 8, 2023: 4pm–6.30pm SGT

Introduction to immunofluorescence staining (User Protocol 10)
Basic image acquisition and quantification
Discussion about your experimental aims and setups

In preparation for this course, you can listen to Yamada’s first webinar about the basic steps of a standard immunofluorescence protocol.


Shuntaro Yamada
Center for Translational Oral Research, University of Bergen

Shuntaro is a D.D.S/Ph.D. candidate at the Center for Translational Oral Research in the Tissue Engineering Group at the University of Bergen. His research interests include the mechanical regulation of stem cell fate for bone tissue regeneration. In addition, he has extensive experience and expertise in immunofluorescent staining and imaging of cells and embryonic or postnatal tissues. Check out Shuntaro’s Instagram Channel for his beautiful immunofluorescence cell images: @stemcell_art

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