Human HepG2 Hepatocellular Carcinoma Cells

HepG2 is a human cell line isolated from a hepatocellular carcinoma of 14 year old male donor. HepG2 cells are used in a wide range of studies, from the oncogenesis to the cytoxicity of substances on the liver

These cells can be used for (i) the study of carcinogenesis as a surrogate for liver toxicity, (ii) the analysis of signaling events, (iii) molecular biology and (iv) protein- based assays. These immortalized hepatic cell lines can be used in place of biopsies for research purposes.

These HepG2 cells are homogenous and grow robustly in cell culture. Vial contains approximately 500,000 cells. Shipped with dry ice.

• Immortalized cell line used as an in vitro model system for liver disease research
• A versatile tool in the fields of hepatology, toxicology and pharmacology
• Typically used to investigate liver diseases caused by incorrect subcellular distribution of cell surface proteins
• Nontumorigenic cells can be conditioned or engineered for mediated cell death

Key Research Applications

• Liver Disease Modeling
• Liver Toxicology Studies
• Cancer Research
• Regenerative Medicine

Cell Characteristics

Property	Details
Growth Properties	Adherent
Donor Age	14 years old
Ethnicity	Caucasian
Gender	Male
Karyotype	Modal number = 55 (range = 50 to 60); has a rearranged chromosome
Tumorigenic	No; No, in immunosuppressed mice; Yes, in semisolid medium
Genes Expressed	alpha-fetoprotein (AFP); albumin; alpha2 macroglobulin; alpha1 antitrypsin; transferrin; alpha1 antichymotrypsin; haptoglobin; ceruloplasmin; plasminogen; complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein; alpha2 HS glycoprotein; beta lipoprotein; retinol binding protein
Expression Markers	Insulin; insulin-like growth factor II (IGF II)

HepG2 carcinoma cells from CET are used as positive control cell lines to study liver cell surface protein interactions in disease modeling, such as non- alcoholic fatty liver disease, and cell-to-cell signaling in engraftment and angiogenesis applications.