Human CD34+ Induced Pluripotent Stem Cells (iPSCs)

Cryopreserved vial of human cord blood isolated CD34+ iPS cells (~500,000 cells)

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  • Introduction

    CD34+ cells isolated from cord blood were reprogrammed with our patented episomal, virus-free method using a proprietary mix of vectors, excluding l-Myc, c- Myc, and Lin28 transcription factors.

    The cell line was validated for pluripotency based on colony morphology, alkaline phosphatase expression, and expression of SSEA-4. Cells are free of Mycoplasma and exhibit classical morphology and growth characteristics.

    CD34+ cells contain hematopoietic stem and progenitor cells and are more naive than similar cells derived from other tissue sources. They are reported to have higher proliferative, engraftment, and multipotent potential. These cells can be used in immunology, stem cell biology, and cell therapy research applications.

    Vial contains approximately 500,000 cells. Shipped with dry ice.

    • Ethically sourced, healthy stem cells reprogrammed from Human CD34+ cells using our patented method
    • Ready-to-use, fast growing cell line with 20 hour doubling times
    • Higher proliferative, engraftment, and pluripotent potential versus other tissue sources
    • Clean karyotype without genetic deletions, insertions or translocations
    • Programmed without oncogenic c-Myc or Lin28 factors to ensure downstream safety

    Key Research Applications

    • High Throughput Screening
    • Disease Modeling
    • Toxicology Testing
    • Tissue Engineering

    Human CD34+ normal progenitor cells normal progenitor cells differentiated into definitive endoderm cells and mature beta cell markers


    CD34+ derived definitive endoderm cells

    CD34+ comparative quantitation


    CD34+ iPSC Genetic Karyotyping


    CD34+G banded metaphase spreads

    CD34+ Genetic Karyotyping

    Description

    Stability & Storage Storage Temperature Storage Time
    Human CD34+ iPS Cells -80°C (preferably in the vapor phase of a liquid nitrogen storage unit) 12 months
    Human iPSC Base Media (not included) 4°C 3 months
    Complete media (see Media Formulation Instructions on documentation) 4°C 14 days
    Human iPS Growth Supplement (not included) -20°C Not applicable (use entire contents)

    Avoid repeated freeze-thaw cycles for cells. Avoid repeated exposure to room temperature and light for medias.

    Click here for the product brochure.

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