The CORALL Total RNA-Seq Library Prep Kit enables fast and cost-efficient generation of UMI labelled, stranded libraries for whole transcriptome analyses using Illumina® NGS platforms.
CORALL is Lexogen’s new stranded total RNA library prep kit with excellent whole transcriptome coverage. CORALL enables streamlined generation of Illumina-compatible libraries within 4.5 hours, featuring seamless integration of Unique Molecular Identifiers (UMIs) and exceptional protocol-inherent strand specificity (>99 %). The fragmentation-free protocol uses Lexogen’s proprietary Strand Displacement Stop and Ligation technologies to deliver complete transcript representation, including start and end sites. CORALL Kits are delivered with either Lexogen i7 6 nt Index Sets for single indexing or with Lexogen UDI 12 nt Unique Dual Indexing Sets for Unique Dual Indexing.
CORALL is suitable for all whole transcriptome RNA-seq applications, including:
Gene expression profiling.
Isoform discovery and quantification.
Alternative splicing studies.
SLAMseq metabolic RNA sequencing.