PIF Supplement

The PIF Supplement is used with buffered peptone water (BPW) in food microbiology for the enrichment of Enterobacteriaceae, particularly Salmonella spp. and Cronobacter spp.. It is adapted for matrices with a high level of background flora, such as lactic acid bacteria, bifidobacteria, and other Gram-positive bacteria. It is especially suitable for the detecton of Salmonella spp. and Cronobacter spp. in powdered infant formula with and without probiotics, ingredients for infant formula, and environmental samples of production areas.

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Description

Principle

The PIF Supplement is a special mix of components, which allows the inhibition of background flora and, when associated with BPW, optimized recovery of Enterobacteriaceae, in particular Salmonella and Cronobacter.

Theoretical Composition

The PIF Supplement is supplied as a specific colored mix in 2 g amounts

Reconstitution Ratio

Dissolve 2 g of PIF Supplement in 40 ml of 1:1 ethanol/sterile distilled water solutiondirectly in the plastic vial Mix, heating if necessary, until a homogeneous suspension is obtained

Enrichment Protocol - Preparation Table

Storage

Store dehydrated at 2-8 ⁰C in a carefully sealed package in a dry and dark place. The PIF Supplement is stable for 6 weeks at 2-8 ⁰C in a dark place once reconstituted

References

- NF Validation BRD 07/06-07/04

- NF Validation BRD 07/11-12/05

- NF Validation BRD 07/23-01/13

- NF Validation BRD 07/22-05/12

- AOAC 082003

User Guide

Safety Data Sheet