PCA (Plate Count Agar) without Dextrose Agar medium, also known as Yeast Extract Agar (YEA), for the enumeration of waterborne microorganisms using an aerobic colony count at 22 °C and 36 °C according to ISO Standard.
Principle
The medium contains tryptone and yeast extract which promote the growth of most bacteria.
Theoretical Composition
| Tryptone | 6 g |
| Yeast extract | 3 g |
| Agar | 12 g |
| Destilled water | 1,000 ml |
| Final pH at 25 ⁰C = 7.2 ± 0.2 |
Reconstitution Ratio
21 g/L (500 g of powder makes 23.8 L of medium)
Storage
Store dehydrated medium at 15-25 ⁰C in carefully sealed bottles in a cool, dry place
References
- ISO 6222:1999
- ISO 11133:2014
- ISO 11133/A2:2020