Dual-Range™ BCA Protein Assay Kit is the most used protein assay method. It based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein in the 5–2,000 μg/mL concentration range. Dual-Range™ BCA Protein Assay Kit is compatible with many detergents but not compatible with reducing agents such as DTT,DTE, and 2- Mercaptoethanol etc.
Blot, ELISA, Immunoassay,
Dual-Range™ BCA Protein Assay Kit is the most used protein assay method. It based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein in the 5–2,000 μg/mL concentration range. Dual-Range™ BCA Protein Assay Kit is compatible with many detergents but not compatible with reducing agents such as DTT,DTE, and 2- Mercaptoethanol etc.
The BCA protein assay is based on a reduction of Cu2+ to Cu+ by proteins in alkaline solution with a sensitive and specific colorimetric detection of Cu+ by bicinchoninic acid (BCA). Two molecules of BCA chelate with each Cu+ and making the reduced copper from apple green to purple complex with strong absorbance at 562 nm.
