Buffered Peptone Water Plus
Buffered Peptone Water (BPW) Plus is used as diluent and nutritive enrichment broth for the
resuscitation and growth of a wide variety of microorganisms including
Buffered Peptone Water (BPW) Plus is used as diluent and nutritive enrichment broth for the
resuscitation and growth of a wide variety of microorganisms including
Principle
The special selection of peptones allows a very high level of nutritivity to ensure the best recovery. In combination with the phosphate buffer's maintenance of a steady pH and osmotic balance, thanks to the presence of chloride sodium, BPW Plus offers favorable conditions for the growth of a wide range of microorganisms from a variety of matrices.
Theoretical Composition
| Peptone | 10 g |
| Sodium chloride | 5 g |
| Potassium dihydrogenphosphate | 1.5 g |
| Disodium hydrogen phosphate | 3.5 g |
| Distilled water | 1,000 ml |
| Final pH at 25 ⁰C = 7.0 ± 0.2 |
Reconstitution Ratio
20 g/L (500 g of powder makes 25 L of medium)
Storage
Store dehydrated medium at 15-25 ⁰C in carefully sealed bottles in a dry and dark place
References
- ISO 11290-2:2017
- ISO 19250:2013
- ISO 21528-1:2017
- ISO 22964:2017
- ISO 6579-1:2017
- ISO 6887-1:2017
- ISO 6887-5:2010