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Mirus: Get your free transfection reagent sample here

Designed to enhance delivery of packaging and transfer vectors to adherant & suspension HEK 293 cell types to increase recombinant AAV & lentivirus production

TransIT-VirusGEN Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures.

TransIT-X2

Versatile transfection reagent that is the solution for hard-to-transfect & common cell types. Avoid guesswork and get it right the first time!

TransIT-X2 Transfection Reagent Enables Superior Transfection in a Variety of Cell Types.

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Fab-TACS® Trial Kit Request

Traceless Affinity Selection

The Fab-based traceless affinity cell selection (Fab-TACS®) technology represents an affinity chromatography system for non-magnetic isolation of exosomes. It is based on Twin-Strep-tagged CD-speci c Fab fragments (Fabs), which reversibly capture and release the exosomes. The technology delivers label-free exosomes with intact biological functions in a standardized manner with highly reproducible quality. The exosomes can be isolated from different cell culture supernatants, serum and plasma.

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Simple Workflow

Columns for exosomes are filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged low affinity Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, cell culture supernatants, serum, plasma or other preparations pass through the column. Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to the target. Unwanted material is efficiently washed away. In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps due to the higher affinity of biotin to Strep-Tactin®. After elution, the Fab-Streps self-dissociate from the vesicle surface. The label-free authentic exosomes are now ready for further downstream applications.In case ultra-pure exosome preparations without Biotin and Fabs are required, size exclusion chromatography or hydrostatic filtration dialysis can be performed.

High Quality Exosme Isolation

Minimal contamination by other EVs

The particle size is a critical factor in evaluating the quality of isolated exosomes. 99% of particles isolated from cell culture supernatants of mesenchymal stem cells (MSCs) fell within the range of 30 - 150 nm (A). This indicates strong exosome enrichment. In comparison, only 32% exosome-sized particles were detected after puri cation with a commercially available PEGylation kit (B), implying contamination by larger extracellular vesicles.

High content of exosome proteins

Since other same-sized non-exosome contaminants may contribute to the pool of isolated particles, the vesicles purified by our Fab- TACS® isolation technology from MSC supernatants were tested for the presence of marker proteins CD63 and Alix. Both proteins were clearly present within the purified particles (C), confirming their exosome phenotype.

Reproducible results

Depending on MSC donor, cell culture supernatant compositions may vary. Purified exosomes of three independent isolations from different MSC donors were very comparable in their size ranging from 86 nm to 90 nm average diameter. All isolations yielded around 94% of particles between 30 and 150 nm in size (D). Besides high purity, this demonstrates a high reproducibility of our Fab-TACS® exosome isolation technology.

Source-independent application

Important exosomes sources also include a variety of human bodily fluids such as blood and urine. Similar to isolations from cell culture supernatants, 96% of particles purified from human serum exhibited the typical exosome size of 30 to 150 nm (E).

Quick and simple

Due to the surface protein specificity of the Fab-TACS® isolation technology, time-consuming centrifugation steps or extensive sample preparations are not necessary. The protocol is easy and straight-forward, permitting efficient and high quality exosome isolation also for newcomers in the field of exosome research. Additionally, the short processing time minimizes the stress of cargo contained within the exosomes.

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Ibidi: Get your sample here

3 Reasons to Use the ibidi Chambers

1.	High-resolution imaging
	The ibidi slides are ideal for a variety of microscopic techniques.
2.	Fast and simple handling
	The all-in-one chambers simplifying your protocol.
3.	Cost-effective experiments
	You only need a small number of cells and low reagent volumes.

Tailored For your Assay:

Choose From 3 Unique Solutions

Chambered Coverslips

Channel Slides

Chamber Slides, Removable

Cell culture and IF in the same well

Homogeneous cell distribution and small medium amounts

Ideal for long-term storage

Bottom	ibidi Coverslip	ibidi Coverslip	Glass slide
Microscope	Inverted	Inverted	Inverted & upright
Storage	Short-term	Short-term	Long-term

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AUM Biotech: FANA Oligos Promotion

Don't miss our exclusive promotions

Free Scamble Control

with every purchase of set of 4 FANA Oligos

4 FANAs per mRNA Knockdown (1 Target) at 10 nmoles

Free 1 SCR Control at 10 nmole

4 FANAs per mRNA Knockdown (1 Target) at 25 nmoles

Free 1 SCR Control at 25 nmole

4 FANAs per mRNA Knockdown (2 Target) at 10 nmoles each

Free 1 SCR Control at 25 nmole

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Why use FANA Oligo For RNA Silencing

The Basics

Transfection Reagents	Not Required
Specificity	FANA proprietary technology allows very high specificity for the target RNA
RISC Associated Off Targetting	None

Ease of Use in Different Model Systems

Basic Cell Line, Primary Cells & Difficult to Transfect Cells	Easy to use. No transfection reagents required. Mix FANA oligos with the cells
Fish Models	Can work in fish models (example zebrafish)
Transition from Basic Cell Lines to Primary Cells to Animal Models	Easy and convenient single step process

Key Attributes

Toxicity	Non-toxic
Stability	Resistant to degradation by serum and cellular nucleases
Resources and Time Saving	Minimal resources needed and easy to Optimize
Cost Savings	Up to 80% more cost efficient in terms of time and money

FANA Treatment Workflow