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Mirus: Get your free transfection reagent sample here

TransIT-VirusGEN
Designed to enhance delivery of packaging and transfer vectors to adherant & suspension HEK 293 cell types to increase recombinant AAV & lentivirus production

TransIT-VirusGEN Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures.

 
TransIT-X2
Versatile transfection reagent that is the solution for hard-to-transfect & common cell types. Avoid guesswork and get it right the first time!

TransIT-X2 Transfection Reagent Enables Superior Transfection in a Variety of Cell Types.

 [button link="https://www.sciencewerke.com/wp-content/uploads/2021/02/Mirus-Sample-Request-form.html" color="red" newwindow="yes"] Get Your Free Sample Today![/button]

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Fab-TACS® Trial Kit Request

Traceless Affinity Selection
The Fab-based traceless affinity cell selection (Fab-TACS®) technology represents an affinity chromatography system for non-magnetic isolation of exosomes. It is based on Twin-Strep-tagged CD-specific Fab fragments (Fabs), which reversibly capture and release the exosomes. The technology delivers label-free exosomes with intact biological functions in a standardized manner with highly reproducible quality. The exosomes can be isolated from different cell culture supernatants, serum and plasma.
Simple Workflow
Columns for exosomes are filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged low affinity Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, cell culture supernatants, serum, plasma or other preparations pass through the column. Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to the target. Unwanted material is efficiently washed away. In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps due to the higher affinity of biotin to Strep-Tactin®. After elution, the Fab-Streps self-dissociate from the vesicle surface. The label-free authentic exosomes are now ready for further downstream applications.In case ultra-pure exosome preparations without Biotin and Fabs are required, size exclusion chromatography or hydrostatic filtration dialysis can be performed.
High Quality Exosome Isolation

Minimal contamination by other EVs

The particle size is a critical factor in evaluating the quality of isolated exosomes. 99% of particles isolated from cell culture supernatants of mesenchymal stem cells (MSCs) fell within the range of 30 - 150 nm (A). This indicates strong exosome enrichment. In comparison, only 32% exosome-sized particles were detected after purification with a commercially available PEGylation kit (B), implying contamination by larger extracellular vesicles.

High content of exosome proteins

Since other same-sized non-exosome contaminants may contribute to the pool of isolated particles, the vesicles purified by our Fab- TACS® isolation technology from MSC supernatants were tested for the presence of marker proteins CD63 and Alix. Both proteins were clearly present within the purified particles (C), confirming their exosome phenotype. 

Reproducible results

Depending on MSC donor, cell culture supernatant compositions may vary. Purified exosomes of three independent isolations from different MSC donors were very comparable in their size ranging from 86 nm to 90 nm average diameter. All isolations yielded around 94% of particles between 30 and 150 nm in size (D). Besides high purity, this demonstrates a high reproducibility of our Fab-TACS® exosome isolation technology.

Source-independent application

Important exosomes sources also include a variety of human bodily fluids such as blood and urine. Similar to isolations from cell culture supernatants, 96% of particles purified from human serum exhibited the typical exosome size of 30 to 150 nm (E).  

Quick and simple

Due to the surface protein specificity of the Fab-TACS® isolation technology, time-consuming centrifugation steps or extensive sample preparations are not necessary. The protocol is easy and straight-forward, permitting efficient and high quality exosome isolation also for newcomers in the field of exosome research. Additionally, the short processing time minimizes the stress of cargo contained within the exosomes.
 

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Ibidi: Get your sample here

3 Reasons to Use the ibidi Chambers
1.
High-resolution imaging
The ibidi slides are ideal for a variety of microscopic techniques.
2.
Fast and simple handling
The all-in-one chambers simplifying your protocol.
3.
Cost-effective experiments
You only need a small number of cells and low reagent volumes.
Tailored For your Assay:
Choose From 3 Unique Solutions
Cell culture and IF in the same well
Homogeneous cell distribution and small medium amounts
Ideal for long-term storage
Bottomibidi Coverslipibidi CoverslipGlass slide
MicroscopeInvertedInvertedInverted & upright
StorageShort-termShort-termLong-term
 

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AUM Biotech: FANA Oligos Promotion

Don't miss our exclusive promotions
Free Scamble Control
with every purchase of set of 4 FANA Oligos
4 FANAs per mRNA Knockdown (1 Target) at 10 nmoles
Free 1 SCR Control at 10 nmole
4 FANAs per mRNA Knockdown (1 Target) at 25 nmoles
Free 1 SCR Control at 25 nmole
4 FANAs per mRNA Knockdown (2 Target) at 10 nmoles each
Free 1 SCR Control at 25 nmole
Why use FANA Oligo For RNA Silencing
The Basics
Transfection Reagents
Not Required
Specificity
FANA proprietary technology allows very high specificity for the target RNA
RISC Associated Off Targetting
None
Ease of Use in Different Model Systems
Basic Cell Line, Primary Cells & Difficult to Transfect CellsEasy to use. No transfection reagents required. Mix FANA oligos with the cells
Fish ModelsCan work in fish models (example zebrafish)
Transition from Basic Cell Lines to Primary Cells to Animal ModelsEasy and convenient single step process
Key Attributes
ToxicityNon-toxic
StabilityResistant to degradation by serum and cellular nucleases
Resources and Time SavingMinimal resources needed and easy to Optimize
Cost SavingsUp to 80% more cost efficient in terms of time and money
FANA Treatment Workflow
Mode of Action
•
FANA oligos uses RNase H-mediated cleavage, eliminating RISC-associated offtarget effects often observed with siRNA
•
FANAs directly targets RNA present within the nucleus
•
FANAs can be self-delivered and do not need transfection reagents or delivery agents
 

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