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Mirus: Get your free transfection reagent sample here

TransIT-VirusGEN
Designed to enhance delivery of packaging and transfer vectors to adherant & suspension HEK 293 cell types to increase recombinant AAV & lentivirus production

TransIT-VirusGEN Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures.

 
TransIT-X2
Versatile transfection reagent that is the solution for hard-to-transfect & common cell types. Avoid guesswork and get it right the first time!

TransIT-X2 Transfection Reagent Enables Superior Transfection in a Variety of Cell Types.

 [button link="https://www.sciencewerke.com/wp-content/uploads/2021/02/Mirus-Sample-Request-form.html" color="red" newwindow="yes"] Get Your Free Sample Today![/button]

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Fab-TACS® Trial Kit Request

Traceless Affinity Selection
The Fab-based traceless affinity cell selection (Fab-TACS®) technology represents an affinity chromatography system for non-magnetic isolation of exosomes. It is based on Twin-Strep-tagged CD-speci c Fab fragments (Fabs), which reversibly capture and release the exosomes. The technology delivers label-free exosomes with intact biological functions in a standardized manner with highly reproducible quality. The exosomes can be isolated from different cell culture supernatants, serum and plasma.
Simple Workflow
Columns for exosomes are filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged low affinity Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, cell culture supernatants, serum, plasma or other preparations pass through the column. Exosomes adhere to the matrix based on the exclusive binding of the Fab-Strep to the target. Unwanted material is efficiently washed away. In a final step, the addition of biotin causes the elution of the exosomes and the Fab-Streps due to the higher affinity of biotin to Strep-Tactin®. After elution, the Fab-Streps self-dissociate from the vesicle surface. The label-free authentic exosomes are now ready for further downstream applications.In case ultra-pure exosome preparations without Biotin and Fabs are required, size exclusion chromatography or hydrostatic filtration dialysis can be performed.
High Quality Exosme Isolation

Minimal contamination by other EVs

The particle size is a critical factor in evaluating the quality of isolated exosomes. 99% of particles isolated from cell culture supernatants of mesenchymal stem cells (MSCs) fell within the range of 30 - 150 nm (A). This indicates strong exosome enrichment. In comparison, only 32% exosome-sized particles were detected after puri cation with a commercially available PEGylation kit (B), implying contamination by larger extracellular vesicles.

High content of exosome proteins

Since other same-sized non-exosome contaminants may contribute to the pool of isolated particles, the vesicles purified by our Fab- TACS® isolation technology from MSC supernatants were tested for the presence of marker proteins CD63 and Alix. Both proteins were clearly present within the purified particles (C), confirming their exosome phenotype.

Reproducible results

Depending on MSC donor, cell culture supernatant compositions may vary. Purified exosomes of three independent isolations from different MSC donors were very comparable in their size ranging from 86 nm to 90 nm average diameter. All isolations yielded around 94% of particles between 30 and 150 nm in size (D). Besides high purity, this demonstrates a high reproducibility of our Fab-TACS® exosome isolation technology.

Source-independent application

Important exosomes sources also include a variety of human bodily fluids such as blood and urine. Similar to isolations from cell culture supernatants, 96% of particles purified from human serum exhibited the typical exosome size of 30 to 150 nm (E). 

Quick and simple

Due to the surface protein specificity of the Fab-TACS® isolation technology, time-consuming centrifugation steps or extensive sample preparations are not necessary. The protocol is easy and straight-forward, permitting efficient and high quality exosome isolation also for newcomers in the field of exosome research. Additionally, the short processing time minimizes the stress of cargo contained within the exosomes.
 

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Ibidi: Get your sample here

3 Reasons to Use the ibidi Chambers
1.
High-resolution imaging
The ibidi slides are ideal for a variety of microscopic techniques.
2.
Fast and simple handling
The all-in-one chambers simplifying your protocol.
3.
Cost-effective experiments
You only need a small number of cells and low reagent volumes.
Tailored For your Assay:
Choose From 3 Unique Solutions
Cell culture and IF in the same well
Homogeneous cell distribution and small medium amounts
Ideal for long-term storage
Bottomibidi Coverslipibidi CoverslipGlass slide
MicroscopeInvertedInvertedInverted & upright
StorageShort-termShort-termLong-term
 

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AUM Biotech: FANA Oligos Promotion

Don't miss our exclusive promotions
Free Scamble Control
with every purchase of set of 4 FANA Oligos
4 FANAs per mRNA Knockdown (1 Target) at 10 nmoles
Free 1 SCR Control at 10 nmole
4 FANAs per mRNA Knockdown (1 Target) at 25 nmoles
Free 1 SCR Control at 25 nmole
4 FANAs per mRNA Knockdown (2 Target) at 10 nmoles each
Free 1 SCR Control at 25 nmole
Why use FANA Oligo For RNA Silencing
The Basics
Transfection Reagents
Not Required
Specificity
FANA proprietary technology allows very high specificity for the target RNA
RISC Associated Off Targetting
None
Ease of Use in Different Model Systems
Basic Cell Line, Primary Cells & Difficult to Transfect CellsEasy to use. No transfection reagents required. Mix FANA oligos with the cells
Fish ModelsCan work in fish models (example zebrafish)
Transition from Basic Cell Lines to Primary Cells to Animal ModelsEasy and convenient single step process
Key Attributes
ToxicityNon-toxic
StabilityResistant to degradation by serum and cellular nucleases
Resources and Time SavingMinimal resources needed and easy to Optimize
Cost SavingsUp to 80% more cost efficient in terms of time and money
FANA Treatment Workflow
Mode of Action
•
FANA oligos uses RNase H-mediated cleavage, eliminating RISC-associated offtarget effects often observed with siRNA
•
FANAs directly targets RNA present within the nucleus
•
FANAs can be self-delivered and do not need transfection reagents or delivery agents
 

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Get your Chemotaxis FastTrack AI Image Analysis for FREE

Are you still manually tracking your cells? ibidi now offers a great way to save time and money!
Receive 30 free Chemotaxis FastTrack AI Image Analysis jobs when you buy a Box of µ-Slide Chemotaxis – for a limited time only*!
The Chemotaxis FastTrack AI Image Analysis is an AI-based automated image analysis solution for single-cell tracking.
•
Accurate cell tracking with powerful artificial intelligence
•
Objective and reproducible analysis without labeling
•
Upload your time-lapse movies and receive analysis results within minutes without manual tracking
Not Already Familiar With Chemotaxis Assays?
Learn more through our Chemotaxis Application site and receive your free sample of the µ-Slide Chemotaxis to get started!
 
*This offer is valid until December 31, 2021 or while supplies last. *Register and key in voucher code found on packaging to redeem Chemotaxis FastTrack AI image Analysis jobs. *For redemption, time-lapse movies will need to be uploaded and results will be produced within minutes or hours.
 

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Omni Exclusive Promotion 2021


Special discounts for all your homogenizing needs
Bead Ruptor Elite
10% OFF
Ideal for isolating DNA, RNA, proteins and small molecules.
•
Process hundreds of samples a day
•
Wide range of accessories
•
No cooldown between runs
OMNI TH PCR Kits.
15% OFF
Accomodates a wide range of lab applications.
•
Handles a wide range of samples from 0.2 mL to 100 mL in volume
•
Process tissue sample in less than 30 seconds
•
Wide variety of generator probe for flexibility in application requirement
OMNI 2 mL Bead Kits
Get 1 Kit Free With Every Purchase of 10 Bead Kits
Bead beating is the most effective and verstile lysis method available
•
Pre-filled with lysing matrix
•
Certified RNase- and DNase free
•
Designed for high forces generated on Bead Ruptor Bead Mill
*Terms and Conditions:
1.
2.
Promocode (OMIP21) must be quoted upon order to take part in the promotion.
Promotions are only applicable for the purchase of Omni Bead Ruptor Elite, TH PCR Kits and 2 mL Bead Kits only.
3.
Promotions may not be combined or used in conjunction with any other discounts, offers, or promotions.
4.
Offer valid until June 30, 2021. Offer valid in Singapore only. Other restrictions may apply. Void where prohibited.
 

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Little Lunatic Trade-in Promotion 2021

TRADE-IN YOUR UV-VIS SPECTROPHOTOMETER AND UPGRADE TO LITTLE LUNATIC
Going smaller
Little Lunatic is there for labs that have fewer samples and lower requirements. It uses a chip-based version of the Lunatic plate to get quick concentration measurements of up to 16 samples in 5 minutes. Researchers that just need a quick check of concentration can get what they need from this little guy.
Simple Workflow
Skip the sample prep, cleaning and worrying about cross-contamination or evaporation – your samples will sit tight for up to 2 hours. Our Lunatic chips runs 16 samples in 5 minutes. Each microfluidic circuit has two fixed pathlengths built-in to cover a wide dynamic range of 0.03-275 OD. All you do is load up your samples and get kick-ass results.
Max out your biologic
Lunatic is the only system out there that can measure biologics at high-throughput and high concentration. It’s got dynamic range that covers from 0.02 mg/mL to 200 mg/mL (mAb), so run any protein without ever having to dilute again.Stop the madness of running just one protein at a time and cleaning up afterwards.
Dig up your genomic's dirt
Lunatic analysis software lets you see annoying impurities that classic A260 measurements alone mistake as your DNA or RNA. Find out exactly how much RNA, turbidity, beads and other influencers are screwing up your nucleic acid quantification.Ditch the time-sucking, dye-based prep and know right away if your samples are good to go.
Ridiculously good data
Get spot-on precision within 1% and accuracy within 2%. Using two fixed pathlengths, Lunatic gets you jaw-dropping data at both low and high protein concentrations and nails the expected concentrations every time. With the smallest sample size, the highest throughput and crazy accurate results, Lunatic is hands down the best tool out there for protein quantification.
Accuracy you can show off
Lunatic has shocking accuracy and wants to prove it. Using the Fundamentals tryptophan standards, the accuracy, precision and linearity of the instrument can be proven at any time — at the protein-relevant 280 nm wavelength and OD range of 20-225. Be confident about every sample with data that leaves no room for doubt.
Downstream ready
Smash through the USP & Ph. Eur. UV/Vis requirements for absorbance accuracy, precision, linearity, wavelength accuracy, stray light and resolution with performance verification measurements of independently certified NIST standards. Lunatic is ready when you are to get validated and make the move into QC.
Terms& Conditions
•
Trade-in is available to Fluorometer and UV-Vis Spectrophotometer of any brand
•
Trade-in Promotion is only valid from 1 May 2021 to 31 December 2021
•
Sciencewerke Pte Ltd reserves the right to amend, delete or add to any terms and conditions on this trade-in promotion from time to time without prior notice
 

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10% Off Norgen's Preservation and Purification Kits

FOR A LIMITED PERIOD, RECEIVE 10% OFF THE FOLLOWING PRESERVATION AND PURIFICATION KITS
Sample Collection and Preservation
cf-DNA/cf-RNA Preservation Tubes
Cat. 639650/Dx63950
For whole blood collection and preservation of cell-free circulating DNA and RNA
Isolation and Purification
Plasma/Serum RNA Purification Kits
Cat. 55000/56100/56200
For rapid and simple purification of circulating RNA and Exosomal RNA from plasma or serum samples
Plasma/Serum Cell-Free Circulating DNA Purification Kits
Cat. 55500/55100/55600/55800
For rapid and simple purification of all sizes of circulating DNA from plasma or serum samples
Exosomal RNA Isolation Kit
Cat. 58000
For use with Urine Exosome Purification Kits or Plasma/Serum Exosome Purification Kits.
Plasma/Serum Exosome Purification Kits
Cat. 57400/57500/57600
Purification and enrichment of intact plasma/serum exosomes for functional studies
Receive Norgen's Exclusive Merchandise when you complete our survey
making science work for you
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t: +65 6777 1045 | f: +65 6777 3054
enquiry@sciencewerke.com | www.sciencewerke.com
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