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Xenografting of Breast Cancer Cells using VitroGel System

RNF208, an estrogen-inducible E3 ligase, targets soluble Vimentin to suppress metastasis in triple-negative breast cancers. In this research highlight, a joint team of researchers from Korean and Japanese cancer institutes performed a series of in vitro and in vivo experiments to investigate the expression and mode of action of the ring-finger protein RFN208. VitroGel, a xeno-free injectable hydrogel system was used for xenografting of triple-negative breast cancer cells into mice to help cell retention and test tumor growth in response to the upregulation of a ubiquitin-related ligase protein. The results show that RNF208 expression is closely associated with Vimentin-mediated aggressive cancer progression of breast cancer cells. Click here for the full research highlights. Read how VitroGel helps with this research. Do you know that xeno-free VitroGel hydrogels can be used as a Matrigel alternative for 3D cell culture research? Click here to find out more.

3 Reasons Why You Should Cultivate Endothelial Cells Under Flow

Did you know that shear stress, which is the mechanical force generated by blood flow, has a crucial impact on the biology of endothelial cells? Read ibidi’s latest blog article for the 3 good reasons why it is more physiological to cultivate cells under flow conditions. Shear Stress Influences:

Cell Signaling and Gene Expression
Cell Morphology, Cell Structure, and Organization
Cell Function and Behavior

Click here for full article.

A look at IgA responses of COVID-19 patient sera to known human autoantigens.

Studies report a link between COVID-19 disease severity and autoimmunity, with growing evidence showing development of autoantibodies as a result of long-lasting or life threatening cases of SARS-CoV-2 infection. Discover the new PEPperCHIP^® Autoimmune Human Epitope Microarray with 3,723 human B-cell epitopes associated with autoimmune diseases. The microarray enables you to:

Monitor autoantibody development in COVID-19 patient sera
Identify autoantibody responses after COVID-19 infection
Investigate autoantibody responses before and after COVID-19 vaccination
Correlate IgG or IgA autoantibodies with disease progression
Analyze autoantibody responses in animal models on the epitope level

[button link="https://www.pepperprint.com/fileadmin/user_upload/mds-new/PEPperCHIP_Autoimmune_Human_Epitope_Microarray2.pdf" color="green" newwindow="yes"] Learn more[/button]

Quantifying antibody-mediated neutralization of SARS-CoV-2 directly in serum

New Scientific Publication

The emergence of mutant variants of SARS-CoV-2 and associated concerns about the effects of these mutations on the efficacy of vaccines and therapeutics has underscored the vital importance of understanding the antibody response to SARS-CoV-2. In our recent paper "In vitro measurements of protein–protein interactions show that antibody affinity governs the inhibition of SARS-CoV-2 spike/ACE2 binding in convalescent serum" we showcase the unique capabilities of our Fluidity One-W Serum platform and describe:

•

A new type of in-solution receptor-binding competition assay that quantifies the affinity, concentration, and neutralization potential of antibodies against the SARS-CoV-2 spike protein

•

The ability of this platform to accurately and quantitatively profile the underlying protein interactions directly in serum

Read the paper

If you have any questions about our technology or would like to see how the unique capabilities of the Fluidity One-W Serum can support your research, please get in touch and we will be happy to discuss your projects with you.

Enquire Now

SARS-CoV-2 & Flu Multiplexed Solutions

Confidently Stratify COVID-19 and Flu Patients

Whether your laboratory is utilizing multiplex or sequential molecular testing workflows, LGC SeraCare offers AccuPlex™ Quality Solutions with targets for SARS-CoV-2, influenza A/B and respiratory syncytial virus (RSV). Available as Verification Panels optimized for assay verification at installation and Reference Material Kits designed to monitor day-to-day assay performance, together these products offer an ideal solution for clinical laboratories looking for a safe and effective tool to verify and monitor molecular assay performance.

[button link="https://www.seracare.com/SARS-CoV-2/sars-cov-2-multiplex/?utm_campaign=Coronavirus%20Launch%20March%202020&utm_medium=email&_hsmi=97830093&_hsenc=p2ANqtz--rc_dTyK6Mz1CHqFQjp5KsGJ_z4gR3oxHt6VoMjRI5fmdHv3-e_tDv5wl82nTdxTQQ33OulrWM107KSbNvWzdwtWrElA&utm_content=97830093&utm_source=hs_email" color="orange" newwindow="yes"] Learn more[/button]

AccuPlex Quality Solutions are:

Non-infectious and replication deficient, enabling safe handling in contrast to viral samples
Fully-extractable with a real viral protein coat; superior to “naked” transcribed RNA by offering challenge to the entire molecular test procedure
Optimized for assay verification and day-to-day performance monitoring
Stable for 2 years at 2-8°C

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Zeesan Complete COVID-19 Workflow

Introducing Zeesan’s complete COVID-19 workflow from point of collection to extraction and detection.

The Saliva RNA Sample Collection Kit provides an all-in-one system for the collection, stabilization and transportation of saliva sample for RNA preservation. [button link="https://www.sciencewerke.com/all_products/saliva-sample-collection-kit/" newwindow="yes"] Find out more[/button]

Zeesan's Automatic and Manual Extraction Kit is intended to be used by professional users, such as technicians and physicians who are trained in molecular biological techniques. The kit provides high-quality RNA that is suitable for downstream applications, such as amplification or other enzymatic reactions. Intended Use: 1) NMPA approved in China for clinical use 2) CE marked for IVD use in Europe [button link="https://www.sciencewerke.com/all_products/virus-rna-extraction-kit/" newwindow="yes"] Find out more[/button]

The Zeesan SLAN^® -96S real-time PCR system is intended for: 1. Clinical diagnosis in vitro; 2.General laboratory use. The Zeesan SLAN^® -96S real-time PCR system is used exclusively within a laboratory and its users should be trained in PCR technology and instrument operation and be familiar with related operations. Intended Use: 1) CFDA approved in China for clinical use 2) CE marked for IVD use in Europe [button link="https://www.sciencewerke.com/all_products/slan-96-pcr-system/" newwindow="yes"] Find out more[/button] [button link="mailto: enquiry@sciencewerke.com" type="big" color="orange" newwindow="yes"] Enquire now[/button]

Zeesan Saliva Sample Collection Kit

Innovative: all-in-one systemfor the collection, stabilization, and transportation of saliva sample for DNA /RNA preservation. Secure: Preservation solution is non-toxic and leakage-proof. Easy to use: only 3 steps to collect DNA/RNA sample. IVD use: CFDA approval for clinical use in China, CE marked for IVD use in Europe.

Features	Specifications
Volume	for collecting 2mL saliva
Customization	available
DNA isolation kits and systems	compatible
Storage	15°C ~ 30°C
Shelf Life	5 years (collected saliva sample is stable for one year)

[button link="https://www.sciencewerke.com/all_products/saliva-sample-collection-kit/" color="green" newwindow="yes"] Enquire now[/button]

2019-nCoV (SARS-CoV-2) ELISA KITS

SignalChem has developed a diverse range of high-quality ELISA kits to serve the scientific community and its broad range of research interests. Our standard ELISA kits come with important biochemical detection tools such as an immobilized antigen, horseradish peroxidase (HRP) conjugated detection antibody, colorimetric substrate solution, and a kit specific manual. SignalChem follows strict quality control processes and all of our kits are manufactured and validated by our scientists in Canada. We also offer these kits as a screening service, which is an economical and convenient approach to the drug discovery continuum.

SignalChem's ELISA Kits can be used for the detection and quantification of SARS-CoV-2 spike protein (RBD) and human anti-SARS-CoV-2 spike protein IgG in biological and recombinant samples.

IVD companies can make use of SignalChem’s high quality (sensitivity and specificity) test as a proof of concept to develop accurate diagnostic tests to detect the SARS-CoV-2.

Catalog Number	Product Name
C19SD-876	2019-nCoV S1 Protein ELISA Kit
C19S1-877	2019-nCoV S1 Human IgG ELISA Kit

[button link="https://www.sciencewerke.com/all_products/signalchem-2019-ncov-sars-cov-2-elisa-kits/" newwindow="yes"] Click here for more information[/button]

PrimeStore® MTM

PrimeStore MTM is intended for the stabilisation, transportation and inactivation of infectious unprocessed nasal washes suspected of containing COVID-19 RNA from human samples.

FDA Cleared Sample Collection Device for Safer, Faster, No Cold Chain Shipping and Testing

• PrimeStore® MTM is FDA cleared for nasal specimens and sputum, core specimens for coronavirus testing

• PrimeStore® MTM is cleared for the inactivation of microbes in both nasal specimens and sputum eliminating need for hazardous shipping packaging and handling

• PrimeStore® MTM is cleared for stabilization of RNA for up to 8 days at ambient temperature, eliminating cold chain shipping requirements

Device discription

The PrimeStore MTM device consists of a storage tube with an O-ring and lip seal containing 1.5 mL of the stabilization solution. These components are intended to inactivate viruses and microbials, lyse cells, disrupt/lyse lipid membranes, denatures proteins, inactivates enzymes, and stabilize RNA and DNA. The transport device is designed for storage of specimens between 2-25 °C. PrimeStore® MTM is compatible with a wide range of spin column and bead based extraction systems providing high quality RNA, DNA, mRNA for molecular analysis. [button link="https://www.sciencewerke.com/all_products/longhorn-vaccines-and-diagnostics-primestore-mtm/" color="orange" newwindow="yes"] Find out more[/button]

Logix Smart™ Coronavirus 2019 (COVID-19) Test Kit

The Logix Smart™ Coronavirus Disease 2019 (COVID-19) Test kit is an in vitro diagnostic test that uses our patented CoPrimer™ technology for the qualitative detection of the RNA from SARS-CoV-2 coronavirus (COVID-19).

Ready-to-use Master Mix, complete with RNaseP internal positive control to verify sample quality
Positive Control (PC), to verify the performance of the master mix
Nuclease-Free Water as a negative control, to verify master mix is free from contamination

Intended Use

SARS-CoV-2 is the virus that causes COVID-19, a contagious, zoonotic disease that causes respiratory infection varying from common cold symptoms to severe pneumonia and occasionally death. The Logix Smart Coronavirus Disease 2019 (COVID-19) test is a real-time RT-PCR test using a proprietary technology called CoPrimers™ (Satterfield, 2014)(Poritz & Ririe, 2014) intended for the qualitative detection of nucleic acid from the SARS-CoV-2 in lower respiratory tract fluids (e.g. bronchoalveolar lavage, sputum, tracheal aspirate), and upper respiratory tract fluids (e.g. nasopharyngeal and oropharyngeal swabs) from individuals with signs and symptoms of infection who are suspected of having COVID-19. The test is available to laboratories certified under the Clinical Laboratory Improvement Amendments of 1988 (CLIA), 42 U.S.C. §263a, to perform high complexity tests, or by similarly qualified non-U.S. laboratories. Negative results do not preclude SARS-CoV-2 infection and should not be used as the sole basis for patient management decisions. Negative results must be combined with clinical observations, patient history, and epidemiological information. The Logix Smart Coronavirus Disease 2019 (COVID-19) is intended for use by trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures. The Logix Smart Coronavirus Disease 2019 (COVID-19) test is only for use under the Food and Drug Administration’s Emergency Use Authorization.

References for the above citations:

Poritz, M., & Ririe, K. (2014, Mar). Getting things backwards to prevent primer dimers. Journal of Molecular Diagnosis, 159-62. doi:10.1016/j.jmoldx.2014.01.001 Satterfield, B. (2014, Mar). Cooperative primers: 2.5 million-fold improvement in the reduction of nonspecific amplification. Journal of Molecular Diagnosis, 163-73. doi:10.1016/j.jmoldx.2013.10.004

Regulatory Clearance

In February 2020, the Logix Smart COVID-19 Test kit received CE marking approval, the principle regulatory clearance allowing the test to be sold as an in vitro diagnostic (“IVD”) for the diagnosis of COVID-19 in European Union states and other markets that accept a CE-IVD mark as valid regulatory approval. On April 3 2020 the Logix Smart COVID-19 Test kit obtained Emergency Use Authorization (EUA) by the Food and Drug Administration (FDA) to be used for the diagnosis of SARS-CoV-2.

[button link="https://www.sciencewerke.com/all_products/co-diagnostics-logix-smart-coronavirus-2019-covid-19-test-kit/" color="red" newwindow="yes"] Find out more[/button]

Norgen's complete COVID-19 workflow

Introducing Norgen's complete COVID-19 workflow from point of collection to quick and easy real time RT- PCR detection based on TaqMan™ technology.

Preserve total RNA including viral RNA with Norgen’s convenient all-in-one collection system for up to 2 months

No need to immediately process samples
Preserve total DNA, including viral DNA, at room temperature 2 years
Preserve total RNA, including viral RNA, at room temperature for 2 months
Inactivate microorganisms and viruses for safe and easy transport and handling
Compatible with most DNA and RNA isolation methods
Compatible with many sample types, including nose, mouth, throat and skin
Validated for COVID-19 detection

[button link="https://www.sciencewerke.com/all_products/sample-collection-and-preservation-kit/" color="teal" newwindow="yes"] Learn more[/button]

For rapid purification of total RNA, including microRNA, without phenol

Extract high quality & purity total RNA including miRNA
No phenol step required
Bind & elute all RNA irrespective of size or GC content, without bias
Efficiently extract small RNA irrespective of GC content
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Isolate from a wide variety of specimens
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

[button link="https://www.sciencewerke.com/all_products/rna-purification-kit/" color="red" newwindow="yes"] Learn more[/button]

Detect SARS-CoV-2 specific RNA with real time RT-PCR based on TaqMan™ Technology

Detection kit for SARS-CoV-2, which causes COVID-19 (2019-nCoV)
Ready to use format containing everything required for detection
Available in 1 format for analysis:
For our COVID-19 information brochure click here

[button link="https://www.sciencewerke.com/all_products/norgen-biotek-2019-ncov-taqman-rt-pcr-kit/" color="orange" newwindow="yes"] Learn more[/button]

Watch to find out how saliva can be used as a viable alternative to swabs in the Covid-19 detection workflow.

[video mp4="https://www.sciencewerke.com/mm/wp-content/uploads/2020/04/Saliva-as-a-Viable-Alternative-Sample-Type-for-SARS-CoV-2-Detection-@-3PM.mp4"][/video]

What is Coronavirus: The current pipeline and the vaccines being developed.

Why is it called Coronavirus and what is the difference between COVID-19, MERS and SARS. Read this report to find out more about its origin, transmission, diagnosis and symptoms. Also, explore the current pipeline and up-to-date overview of the development of potential vaccines. [button link="https://www.sciencewerke.com/edm/others/Disease%20Briefing:%20Coronavirus/CORONAVIRUS-REPORT-2.14.2020.pdf" color="red" newwindow="yes"] Download the report here[/button]

Official Distributor for PolyAn GmbH

Sciencewerke Pte Ltd is pleased to announce that we are the official distributors for PolyAn GmbH Our variety of solutions include: Microarray Consumables - PolyAn is one of the leading producers of functionalized substrates for microarrays. Our wide range of surfaces, substrates and handling tools for microarrays enables our customers to select the most suitable substrate for their specific application. Our products include plastic and glass slides, coverslips as well as functionalized plastic films and 96-well plates for DNA-, peptide- or protein microarrays. Additionally, PolyAn is the European distributor of Grace Bio-Labs’ Nitrocellulose slides, SecureSeal and ProPlate product families. With our microarray consumables we enable applications ranging from low-density microarrays for diagnostic tests to ultra-high density arrays for pharma screening. Fluorescent Microparticles (beads, microspheres, sub-micron particles) - PolyAn is offering a portfolio of monodisperse microparticles (beads) and sub-micron particles for agglutination and turbimetric assays, cell interaction studies and instrument calibration. For flow cytometers and fluorescence imaging systems PolyAn offers multiplex bead sets with up to 25 distinct populations. PolyAn’s microparticles can be colour-encoded with a wide range of fluorescent dyes and functionalized with PolyAn reactive 3D-matrices for covalent coupling of biomolecules. Calibration Tools for Fluorescence Imaging Systems - PolyAn’s calibration slides are designed for the routine calibration of confocal fluorescence microscopes and other fluorescence imaging systems. PolyAn’s calibration slides for cell assays are primarily used as quality controls in IVD systems for immunology applications. To find out more about PolyAn GmbH, click here.

Biotium: All-In-One GelRed®

GelRed® Prestain Plus 6X DNA Loading Dye is an agarose gel loading buffer that includes our sensitive, non-toxic, non-mutagenic GelRed® dye. This is an improved version of our original 6X GelRed® prestain loading buffer, formulated to minimize DNA migration shift and increase brightness.

Features:

6X agarose gel loading buffer that includes GelRed® DNA stain.
Convenient: one-step addition of loading buffer and DNA dye to the sample.
Improved formulation to minimize DNA migration shift.
GelRed® DNA stain is an ultra-sensitive, non-mutagenic, non-toxic EtBr alternative.
Two blue tracking dyes that run at ~1.5 kb and ~200 bp in 1% agarose.

[button link="https://www.sciencewerke.com/enquiry/" color="teal" newwindow="yes"] Click here to submit an enquiry [/button]

Official distributor for Phase Genomics

We are pleased to announce that we are the official distributor for Phase Genomics, the leader in Hi-C proximity-ligation based genomic solutions for Southeast Asia! Phase Genomics offers a comprehensive portfolio of laboratory and computational services and products, which are now available at Sciencewerke. Read the press release on Yahoo Finance here For more information on Phase Genomics click here

Sample collection, preservation and isolation

Features and benefits

Fast and easy processing.
No phenol or chloroform, no downstream interference.
Robust, superior performance even with complex samples.
Samples can be preserved at room temperature hence, save on storage and transport.
Stabilize microbial profiles from point of collection.
No precipitation of proteins and loss of valuable information caused by sample freezing/thawing.

Top choices

Enquire with us for more info.

Fixable cell surface stains in many colours

MemBrite™ Fix Cell Surface Stains come with your choice of eight colours from blue to near-IR.

Unique kits allow you to conveniently image cell boundaries
Rapid, uniform live cell surface staining
Covalent labelling can be fixed & permeabilized for IF staining
Stain yeast and gram-positive bacteria
Choose from eight bright & photostable dye colours from blue to near-IR

For more info, click here

Why Single-Cell Westerns?

Many cell populations, such as tumor cells or differentiating stem cells, are heterogeneous. Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. This approach allows simultaneous measurement of multiple proteins in over 1,000 single cells using commercially available Western-validated antibodies.

Milo lets you:

Identify cell subpopulations you can’t see with conventional Westerns
Resolve protein isoforms in individual cells
Identify target expression heterogeneity
Count and quantitate at the same time

Explore how Milo can help you!

Need High Throughput for Your Cell Culture Assays?

High-resolution cell microscopy on a tissue culture-treated surface Brilliant fluorescence imaging without autofluorescence Ready for HTS and robotics: compliant with ANSI / SLAS (SBS)

µ-Plate 24 Well, µ-Plate 96 Well, µ-Plate Angiogenesis 96 Well

	Available in October, 2018: The ibidi µ-Plate 384 Well with clear bottom and clear walls. Check out our website for more information soon.
Perform High-Throughput Screenings With Reproducible Results
	μ-Plate 24 Well\| μ-Plate 96 Well Flat ibidi Polymer Coverslip bottom for high-resolution live cell imaging Without autofluorescence or well-to-well crosstalk for excellent immunofluorescence microscopy
Immunofluorescence staining of MDCK cells.	Ideal for: Live cell imaging Fluorescence microscopy Compound screenings (toxicology) Large-scale transfection assays Wound healing assays using the μ-Plate 24 Well in combination with the ibidi Culture-Inserts
Conduct Angiogenesis Assays and Get Brilliant Cell Visualization
	μ-Plate Angiogenesis 96 Well Well-in-a-well technology for brilliant cell visualization without meniscus Low well volume for cost-effective angiogenesis assays
Tube formation assay of HUVEC.	Ideal for: Live cell imaging Fluorescence microscopy Angiogenesis and tube formation assays High throughput screening of anti-angiogenic drug potential

Latest in high content screening

With Etaluma’s high resolution, versatile, and compact inverted microscope, you can finally get the big picture without the big ticket price!

Lumascope LS720

Surprisingly affordable
Amazingly simple
Remarkably versatile
AUTOMATED. Auto XY stage, autofocus and Z-stacking
VERSATILE. 3-Color fluorescence, phase contrast, brightfield
MULTI-MODE. Records images, time-lapse & live video
HIGH RESOLUTION. Comparable to traditional, high-cost microscopes
SOLID STATE OPTICS. No moving parts, no pixel shift
COMPACT. Fits inside incubators, hoods, and hypoxia chambers for live cell imaging
SIMPLIFIED OPTICS. Captures more light so less cytotoxicity and photo bleaching
FLEXIBLE. Microplates, slides, microfluidic chips, dishes, flasks, custom labware
CAPABLE. Use with 1.25-100x objectives

Send an enquiry below.

Stain mitochondria and lysosomes in live cells

Fluorescent Mitochondrial & Lysosome Stains

Biotium offers bright, non-toxic organelle stains, now with more color options. Highly specific, rapid staining with no wash step required.

MitoView™ Live Cell Mitochondrial Stains

MitoView™ 405 violet-excitable blue stain, now with improved photostability
MitoView™ Green stains live or fixed cells
Far-red MitoView™ 633 senses mitochondrial membrane potential in apoptosis
Unique MitoView™ 720 for far-red or near-infrared imaging

LysoView™ Live Cell Lysosome Stains

LysoView™ 405, violet excitable blue fluorescent probe
Green fluorescent LysoView™ 488, orange fluorescent LysoView™ 540, or far-red fluorescent LysoView™ 633
Light-On LysoView™ 555 - unique UV-activated red lysoview stain

67 Ayer Rajah Crescent #04-21
Singapore 139950

+65 6777 1045

enquiry@sciencewerke.com

Sciencewerke Pte Ltd

67 Ayer Rajah Crescent, #04-21 Singapore 139950 Tel: +65 6777 1045 Fax: +65 6777 3054

HEADQUARTERS SINGAPORE
Sciencewerke Pte Ltd

67 Ayer Rajah Crescent, #04-21
Singapore 139950

Tel: +65 6777 1045
Fax: +65 6777 3054

PT Sciencewerke

Jl. Palmerah Barat 25 Jakarta Barat 11480 Tel: +65 6777 1045 Fax: +65 6777 3054

INDONESIA
PT Sciencewerke

Jl. Pamerah Barat 25 Jakarta Barat 11480

Tel: +62 21 53667591
Fax: +62 21 54844232
WhatsApp: +62 855 1288801
BBM: 74DE155B

Sciencewerke Sdn Bhd

MALAYSIA
Sciencewerke Sdn Bhd

No. 7A, 1st Floor, Jalan Alfa B U6/B, Pusat Perdagangan
Subang Permai, Seksyen U6, 40150 Shah Alam, Selangor,
Malaysia

Tel: +60 3 7846 4244
Fax: +60 3 7847 4244

Sciencewerke (Myanmar) Co., Ltd

MYANMAR
Sciencewerke (Myanmar) Co., Ltd

3 Mahar Swe Condo A-607, No.3, Mahar Swe Street,
13 Ward, Hlaing Township, Yangon

Tel: +95 995517 7717