{"id":2704,"date":"2026-05-04T04:50:52","date_gmt":"2026-05-04T04:50:52","guid":{"rendered":"https:\/\/www.sciencewerke.com\/malaysia\/five-steps-to-successful-cell-membrane-staining-imaging-2\/"},"modified":"2026-07-13T13:14:48","modified_gmt":"2026-07-13T05:14:48","slug":"five-steps-to-successful-cell-membrane-staining-imaging-2","status":"publish","type":"post","link":"https:\/\/www.sciencewerke.com\/malaysia\/?p=2704","title":{"rendered":"A Tech Tip for You: Five Steps to Successful Cell Membrane                                Staining &#038;                                Imaging"},"content":{"rendered":"<figure class=\"project-feature\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1938\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/five-steps-to-successful-cell-membrane-staining-imaging.jpg\" alt=\"\" width=\"903\" height=\"315\" \/><\/figure>\n<p class=\"text-justify\">Tips for success with Biotium\u2019s line of CellBrite\u00ae and MemBrite\u00ae membrane and cell surface stains.<\/p>\n<p class=\"text-justify\">Membrane and cell surface stains are very useful for visualizing cell borders and morphology in multicolour staining of live or fixed cells. Biotium offers several options for cell surface imaging for different applications.<\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>1. Choose the right stain for live or fixed cells<\/strong><\/p>\n<p class=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1937\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/five-steps-to-successful-cell-membrane-staining-imaging1.jpg\" alt=\"\" width=\"940\" height=\"273\" \/><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>2. Understand the workflow before you begin<\/strong><\/p>\n<p class=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1936\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/five-steps-to-successful-cell-membrane-staining-imaging2.jpg\" alt=\"\" width=\"940\" height=\"653\" \/><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>3. Use the right buffer for staining<\/strong><\/p>\n<p class=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1935\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/five-steps-to-successful-cell-membrane-staining-imaging3.jpg\" alt=\"\" width=\"940\" height=\"255\" \/><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>4. Use the right fixative and mounting medium<\/strong><\/p>\n<p class=\"\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1934\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/five-steps-to-successful-cell-membrane-staining-imaging4.jpg\" alt=\"\" width=\"940\" height=\"449\" \/><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>5. Know what to expect when imaging<\/strong><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>Relevant products:<\/strong><\/p>\n<p><a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/product\/cellbrite-cytoplasmic-membrane-dyes\/\" target=\"_blank\" rel=\"noopener\">CellBrite\u00ae Cytoplasmic Membrane Dyes<\/a><br \/>\n<a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/product\/cellbrite-nir-cytoplasmic-membrane-dye\/\" target=\"_blank\" rel=\"noopener\"> CellBrite\u00ae NIR Cytoplasmic Membrane Dyes<\/a><br \/>\n<a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/product\/cellbrite-fix-membrane-stains\/\" target=\"_blank\" rel=\"noopener\">CellBrite\u00ae Fix Membrane Stains<\/a><br \/>\n<a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/product\/membrite-fix-cell-surface-staining-kits\/\" target=\"_blank\" rel=\"noopener\"> MemBrite\u00ae Fix Cell Surface Staining Kits<\/a><\/p>\n<p class=\"\" style=\"font-size: 18px; color: #178fcf;\"><strong>Resources:<\/strong><\/p>\n<p><a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/tech-tips\/tech-tip-cell-surface-stains-for-live-fixed-cells\/?utm_campaign=Tech%20Tips&amp;utm_medium=email&amp;_hsmi=292060667&amp;_hsenc=p2ANqtz-8C0Ouxu9wNdkuzAtZmXj0dT7p2TCu0kTWULfhfi41oFaa6ny7bwf4cCN2J6Dydt6raA5BXGtLEOuz9PYHEyQDcoE15EA&amp;utm_content=292060667&amp;utm_source=hs_email\" target=\"_blank\" rel=\"noopener\">Tech Tip: Cell Surface Stains for Live &amp; Fixed Cells<\/a><br \/>\n<a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/technology\/cellular-stains\/cell-surface-stains\/?utm_campaign=Tech%20Tips&amp;utm_medium=email&amp;_hsmi=292060667&amp;_hsenc=p2ANqtz--qRDBb3QaozYHI9UwW0-IpKda2H-ud4CnX_fR22O8m00L-PR5XisI3PQloflX-K0huAcCpGgn7poduLYVwTuZQv7Ohrw&amp;utm_content=292060667&amp;utm_source=hs_email\" target=\"_blank\" rel=\"noopener\"> Membrane &amp; Surface Stains Selection Guide<\/a><br \/>\n<a style=\"color: #178fcf;\" href=\"https:\/\/biotium.com\/faqs\/category\/cellbrite-cytoplasmic-membrane-stains\/?utm_campaign=Tech%20Tips&amp;utm_medium=email&amp;_hsmi=292060667&amp;_hsenc=p2ANqtz-_NbsRK1rMlecWfxBltMcsiv0ZMK41OVVF7B13PGK31htXG6t-ffO18gb6O5KzRuTfZdSnHsRsWVKVE1f20mowHSJf3lQ&amp;utm_content=292060667&amp;utm_source=hs_email\" target=\"_blank\" rel=\"noopener\">CellBrite\u00ae &amp; MemBrite\u00ae FAQs<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tips for success with Biotium\u2019s line of CellBrite\u00ae and MemBrite\u00ae membrane and cell surface stains. Membrane and cell surface stains are very useful for visualizing cell borders and morphology in multicolour staining of live or fixed cells. Biotium offers several options for cell surface imaging for different applications. 1. Choose the right stain for live [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1938,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[164],"tags":[],"class_list":["post-2704","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-164"],"_links":{"self":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2704","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2704"}],"version-history":[{"count":3,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2704\/revisions"}],"predecessor-version":[{"id":3677,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2704\/revisions\/3677"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/media\/1938"}],"wp:attachment":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2704"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2704"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2704"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}