{"id":2686,"date":"2026-05-04T04:50:50","date_gmt":"2026-05-04T04:50:50","guid":{"rendered":"https:\/\/www.sciencewerke.com\/malaysia\/biotium-tech-tip-2\/"},"modified":"2026-07-13T13:58:10","modified_gmt":"2026-07-13T05:58:10","slug":"biotium-tech-tip-2","status":"publish","type":"post","link":"https:\/\/www.sciencewerke.com\/malaysia\/?p=2686","title":{"rendered":"Biotium Tech Tip"},"content":{"rendered":"<figure class=\"project-feature\"><img decoding=\"async\" class=\"img\" src=\"http:\/\/sciencewerke.local\/wp-content\/uploads\/2026\/04\/img-1-2.webp\" alt=\"\" \/><\/figure>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-1921\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/img-1-2-1024x416.webp\" alt=\"\" width=\"1024\" height=\"416\" \/><\/p>\n<p class=\"text-justify\" style=\"font-size: 20px;\"><strong><br \/>\nTech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst<\/strong><\/p>\n<p class=\"text-justify\">Many researchers are unaware that photoconversion of DAPI and Hoechst can<br \/>\ncause nuclear stains to fluoresce in the FITC and Cy\u00ae3 channels.<\/p>\n<p class=\"text-justify\">Learn about the different ways to avoid DAPI photoconversion.<\/p>\n<p><a style=\"text-decoration: none; color: darkblue;\" href=\"https:\/\/biotium.com\/tech-tips\/tech-tip-avoiding-artifacts-from-uv-photoconversion-of-dapi-and-hoechst\/\"><br \/>\nTech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst<br \/>\n<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-large wp-image-1922\" src=\"https:\/\/www.sciencewerke.com\/malaysia\/wp-content\/uploads\/2026\/04\/img-1-1-1024x652.webp\" alt=\"\" width=\"1024\" height=\"652\" \/><br \/>\n<img decoding=\"async\" src=\"http:\/\/sciencewerke.local\/wp-content\/uploads\/2026\/04\/img-1-1.webp\" alt=\"\" \/><\/p>\n<p class=\"text-justify\" style=\"font-style: italic;\">Photoconversion of DAPI to green and red fluorescence after exposure to<br \/>\nUV<br \/>\nlight. Fixed<br \/>\nand permeabilized HeLa cells stained with DAPI were imaged by confocal microscopy<br \/>\nbefore or after exposure to mercury lamp excitation through the DAPI filter cube for 3<br \/>\nminutes.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst Many researchers are unaware that photoconversion of DAPI and Hoechst can cause nuclear stains to fluoresce in the FITC and Cy\u00ae3 channels. Learn about the different ways to avoid DAPI photoconversion. Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst Photoconversion of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":1921,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[164],"tags":[],"class_list":["post-2686","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-164"],"_links":{"self":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2686"}],"version-history":[{"count":2,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2686\/revisions"}],"predecessor-version":[{"id":3704,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/posts\/2686\/revisions\/3704"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=\/wp\/v2\/media\/1921"}],"wp:attachment":[{"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2686"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.sciencewerke.com\/malaysia\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}