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Uncle

Unchained Labs

Cracking stability using a pile of one-trick, protein-hungry tools is a ton of work. Uncle combines 3 different measurement modes — fluorescence, SLS and DLS. So you can crank out all your data in just a few hours, and use way less protein. All the info you’ll get makes picking the best formulation or protein construct a piece of cake.

Get more data with way less protein. The UNi only needs 9 µL of sample, and you pick how you use it. Run 1 sample in the
morning, 48 in the afternoon. Do a DLS read if that’s all you need. Or, check DLS, then start a 3-day experiment to monitor real-time stability. Your samples are
sealed airtight, so runs can be short or long — your call.

Biologics behave differently. With UNcle, you get the whole fluorescence spectrum, so you don’t need to know ahead of time how your protein behaves. No need to add dyes either. UNcle picks up aggregation on two wavelengths — you’ll see both small and large aggregates no matter what.

Specifications

Instrument
Minimum sample:  volume 9 µL, sealed capillaries
Simultaneous samples per experiment:  48
Sample temperature range: 15–95 °C
Minimum sample concentration:  0.05 mg/mL – 150 mg/mL IgG (protein-dependent)
Heating rate:  0.1–10 °C/minute
Temperature control accuracy:  ±1 °C (<70 °C), ±1.5 °C (>70 °C)
Environmental conditions Temperature range:  18–28 °C
Humidity:  40–60% relative humidity (non-condensing)
Physical:  54 cm W x 50 cm D x 58 cm H, 50 kg
Electrical:  Auto switching power supply, voltage 110–240 V AC, 50–60 Hz, single phase, fuse rating
T6.3AL, 250V, max power 600 W
Regulatory compliance:  Software has optional 21CFR11

Fluorescence and static light scattering
Sample precision:  <2% CV (Tm)
SLS resolution:  ~15 kDa change in mean molecular mass
Excitation:  266 nm and 473 nm laser
Detection:  Fluorescence: CCD spectrometer at full 250–720 nm spectral range SLS: intensity at 266 nm and 473 nm

Dynamic light scattering
Hydrodynamic diameter range:  0.3–1000 nm Size accuracy ± 2%
Minimum sample concentration:  0.1 mg/mL — lysozyme
Molecular weight range: 192 Da – 25 MDa
Light source:  660 nm laser diode
Detection:  Avalanche photodiode module