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Stunner

Unchained Labs

Stunner is the only system that pulls together UV/Vis concentration, Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) data from the same 2 μL sample. Dig in to your AAV by bringing protein and ssDNA concentrations together with light scattering to get the total capsid titer and empty/full ratio. Nail down your protein quality by knocking concentration, hydrodynamic size, polydispersity, and detection of aggregates off your list in one shot. Without skipping a beat, you’ll know if your AAV or protein is good to go.

Teeny sample, tons of info

Just load 2 µL of sample in a Stunner plate — don’t bother with sample prep or dilution. Each microfluidic circuit has two fixed pathlengths built into it to cover a wide dynamic range of 0.03-275 OD. If you’re dealing with a full plate, get 96 concentration measurements in 10 minutes — add on DLS sizing and have both done in an hour. For even heavier workflows, hook it up to your favorite robot to add more oomph.

Know your AAV inside out

Get to the numbers you actually want – titers. Stunner bridges DLS and UV/Vis data to tally up how many full and empty capsids are present, and how much extra protein and DNA is left over. Take your cleaned up AAV and sneak a peek down to 1012 vg/mL. In just one assay, Stunner’s dye-free, label-free, standard-free, hassle-free workflow tells the whole titer story.

Specifications

instrument
Dimensions: 37 cm W x 54 cm D x 33 cm H; 26 kg
Electrical: Universal input voltage 100-240 V AC, 50-60 Hz
Computer: Separate computer with Windows 10 included
Connection: USB, TCP/IP (Service)
Approval: CE, FCC, CSA
Regulatory compliance: Optional 21CFRp11 software package

UV/Vis
Light source: Xenon flash lamp
Detectors: UV/Vis polychromatic spectrophotometer
Wavelength range: 230-750 nm
Wavelength accuracy: ≤400nm: ±1nm; ≥400nm: ±2 nm
Spectral resolution: Better than 2 nm (toluene in hexane)
Absorbance precision (1 cm quartz cuvette): <1 OD: ±0.005 OD st dev 1-2 OD: ±0.5% CV Absorbance accuracy (1 cm quartz cuvette): <1 OD: ±0.01 OD 1-2 OD: ±1%

DLS
Light source: 660 nm laser diode
Detection: Avalanche photodiode module
Size accuracy: ±2%
Minimum sample concentration: 0.1 mg/mL lysozyme
Hydrodynamic diameter range: 0.3–1000nm

Stunner plate specifications
Samples per plate: 96 (12 x 8 microplate format)
Sample retention time: Up to 2 hours
Recommended sample volume: 2 µL
Pathlength(s): 0.1 mm & 0.7 mm path
Measurement time for full plate: ~10 minutes for UV/Vis only; ~1 hour for UV/Vis and DLS

Measurement range
OD 10 mm: 0.03–275 OD 10 mm
ng/µL dsDNA: 1.5–13750 ng/µL
mg/mL ave protein mix: 0.03–275 mg/mL

Absorbance precision (10 mm pathlength): <1 OD: ±0.01 OD st dev 1-200 OD: ±1% CV Absorbance accuracy (10 mm pathlength): <1 OD: ±0.02 OD 1-200 OD: ±2%