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cf-DNA & cf-RNA Preservation tubes

Norgen Biotek

  • Fixative-free preservative, no cross-linking of DNA
  • Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
  • Preserve cf-RNA for 30 days at ambient temperature
  • Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
  • No plasma volume loss after shipping/transportation
  • Prevent hemolysis allowing better separation of plasma
  • Prevent apoptosis of blood cells and fragmentation of genomic DNA
  • Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
  • Vacuumed to draw 8.4 mL of blood in 10 mL tubes

Norgen’s Cell-Free Circulating (CFC) DNA and RNA Purification Kits provide an efficient method for the purification of fragmented free-circulating DNA, RNA, exosomal RNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA or RNA irrespective of size or GC content, without bias. These kits provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA or RNA from large sample volumes. The purified cfc DNA or RNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern blot analysis, reverse transcription PCR, Northern blotting, RNase protection, primer extension and expression array assays

Purification

Norgen’s Cell-Free Circulating (CFC) DNA and RNA Purification Kits provide an efficient method for the purification of fragmented free-circulating DNA, RNA, exosomal RNA or total nucleic acids from variable volumes of plasma/serum or cell-free urine samples. The kits are able to isolate all sizes of circulating DNA or RNA irrespective of size or GC content, without bias. These kits provide an advantage over other available kits in that they do not require extension tubes for the purification of cfc DNA or RNA from large sample volumes. The purified cfc DNA or RNA are eluted in an elution solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR, Southern blot analysis, reverse transcription PCR, Northern blotting, RNase protection, primer extension and expression array assays.

Preservation

Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cell-free circulating DNA/circulating tumor DNA in human whole blood samples during storage and shipping. The cell-free circulating DNA/circulating tumor DNA levels are stable for up to 30 days at room temperature or up to 8 days at 37°C. Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s different Plasma/Serum DNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA for any downstream application assays

Quantification

Since measuring the cfc-RNA and cfc-DNA concentrations from cell-free bodily fluids using common quantification methods is very difficult and challenging due to their low amounts, Norgen has developed two PCR-based quantification kits for the accurate and reliable quantification of cfc-DNA and cfc-RNA from samples that normally contain very low nucleic acid amounts. These kits offer a PCR-based detection procedure to quantify DNA of a wide spectrum of concentrations, including the lower ng per µL, pg per µL and sub-pg per µL range. The unknown DNA or RNA is accurately quantified by using a standard curve constructed from the provided DNA/RNA Standards.