MagStrep “type3” XT beads are magnetic beads consisting of a 6% agarose coated with the streptavidin variant Strep-Tactin®XT and can be used for Strep-tag®II and Twin-Strep-tag® fusion proteins. Due to the magnetic core no centrifugation step is required since the beads can be easily and swiftly separated with a magnetic separator, reducing the risk of disturbing the pellet or loss of beads within the wash step to zero.
As with any other Strep-Tactin®XT resins, the unspecific protein binding is extremely low, which results in an outstanding purity of the target protein. However, MagStrep “type3” XT beads provide the highest binding capacity. These characteristics make them a perfect tool for isolation of proteins or complexes via pull-downs, immunoprecipitation (IP), or co-immunoprecipitation (Co-IP) in batch format and/or high-throughput screenings.
The elution of target proteins or complexes can be triggered under mild conditions with the biotin-containing Buffer BXT or under denaturing conditions by boiling in SDS gel loading buffer. 20 μl suspension correspond to 1 μl magnetic beads.
|Bead Size:||25 μm (average)|
|Possible Application:||Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins|
|Specificity:||Twin-Strep-Tag® and Strep-tag®II|
|Strep-tag®II Binding Capacity:||25.5 mg/ml|
|Support:||6% agarose, crosslinked, spherical magnetic beads|
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