Why Single-Cell Westerns?
Many cell populations, such as tumor cells or differentiating stem cells, are heterogeneous. Protein expression measurements made at the population level often overlook significant differences in expression between individual cells and the existence of cell subpopulations. Milo combines single-cell resolution with the power and versatility of Westerns. This approach allows simultaneous measurement of multiple proteins in over 1,000 single cells using commercially available Western-validated antibodies.
Milo lets you:
- Identify cell subpopulations you can’t see with conventional Westerns
- Resolve protein isoforms in individual cells
- Identify target expression heterogeneity
- Count and quantitate at the same time