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Dye-based qPCR

HighQu

Instrument ORA™ qPCR Green ROX L Mix, 2X

 

ORA™ SEE qPCR Green ROX L Mix, 2X

ORA™ qPCR Green ROX H Mix, 2X

 

ORA™ SEE qPCR Green ROX H Mix, 2X

ORA™ qPCR HRM Mix, 2X
Agilent: Mx3000P®, Mx3005P®, Mx4000P®  ●
Analytic Jena: qTOWER  ●
BioRad: Opticon®, Opticon®2, Chromo4™, MiniOpticon™, CFX96™, CFX384™  ●
BJS: Xxpress®  ●
Cepheid: SmartCycler®
Illumina: Eco
Eppendorf: Mastercycler® ep realplex, Mastercycler® realplex 2S
Fluidigm: BioMark™
Hain Lifescience: FluoroCycler®96
Life Technologies: 7500, 7500 FAST, Viia™7, QuantStudio™ 12K Flex
Life Technologies: 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOnePlus™
QIAGEN: Rotor-Gene®Q, Rotor-Gene® 6000, Rotor-Gene® 3000
Roche Applied Science: LightCycler®480, LightCycler®96, LightCycler®Nano
Takara: Thermal Cycler Dice®
Thermo Fisher Scientific: Piko Real®
Techne: PrimeQ, Quantica®

Benefits

  • Universal – both standard and fast cycling
  • Excellent for GC or AT rich templates
  • Highest sensitivity, rapid extension, early Ct values
  • Supplied with PCR Water

 

highQu qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT and GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the ORA™ qPCR Green Mastermixes are available in different versions –with low or high ROX concentration.

For optional use, the ROX passive reference dye is premixed within the ROX L and ROX H qPCR Mixes. If the purchaser has an instrument capable of optional ROX detection and wishes to perform the optional normalization of the signal, then the user must select the option in the software.

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