HD scRNA-Seq applications
High-Definition single-cell RNA sequencing works best with ultra-low initial inputs (as low as 10 pg RNA). By seeing more genes than classical bulk scRNA-Seq methods, it expands transcriptomic analysis into new scientific applications.
High-definition single-cell differential gene expression analysis
Detailed characterization of cell subpopulations after larger, less sensitive screenings (bulk scRNA-Seq)
Rare cell RNA analysis (e.g., circulating tumor cells (CTCs) or innate lymphoid cells (ILCs))
Subcellular (cytoplasm, organelle) RNA analysis
Single nucleus RNA-Seq
Benefits
LUTHOR HD reaction allows to amplify minuscule amounts of RNA material and to obtain a full understanding of each cell’s gene expression status.
Unmatched sensitivity: See even lowly expressed genes (<10 copies/cell)
UMIs included: Avoid any read count duplicates
Direct RNA amplification: No more gDNA in your reads
Compatible with "less-than-a-cell" inputs: Go below 10 pg
