The BioLector Pro combines the scalable BioLector technology with a microfluidic chip. The system performs high-throughput cultivations in batch or fed-batch mode together with online monitoring and the control of biomass, pH, DO, and fluorescences. All important process features are combined on one single disposable plate.
The system is based on standard microtiter plate formats and operates with non-invasive optical sensors.
The BioLector Pro microbioreactor’s disposable 48-well microtiter plate features online measurements of four parameters and, at the same time, controls pH and feeding rates through micro-valves and micro-channels. These microfluidic components realize continuous feeding and pH control on a microtiter plate format. The feeding modes can be constant, linear, exponential or triggered by signal. Tubing and liquid handlers are no longer needed; everything is part of the gamma-radiated, ready-to-use plate.
By using microfluidic chip technology, extremely exact and slow dosing – down to 50 nL – can be realized. Two reservoir wells per four individually-controllable cultivation reactors are available. Altogether, 32 bioreactor wells and 16 reservoir wells are placed on one plate.
Due to the continuous and rigorous shaking during the optical measurements and throughout the entire experiment, it is ideally suited for aerobic cultures in high density. The massive acquisition of relevant bioprocess data allows precise and reliable bioprocess development, strain characterization, media optimization and clone screening in short time frames.
Similar to the microbioreactor BioLector, additional modules are available for a further functionality upgrade of the BioLector Pro.
The combination of multiple modules is possible.
/// Gas Modules
O2 up regulating module for the up-regulation of oxygen up to 35 %
O2 down regulating module for the down-regulation of oxygen down to 2 %
CO2 up regulating module for fermentation with a CO2 controlled gas atmosphere
Anaerobic cultivation set for cultivating under anaerobic conditions.
/// Fluorescence Module
LED / filter module for fluorescence measurements for wavelengths between 450 nm – 800 nm.
Up to three additional fluorescence modules fit into the system, in addition to the standard filters for biomass, pH, and DO.
For triggered sampling, harvesting or additional feeding, the BioLector Pro can be combined with a liquid handling robotic system. Look at the RoboLector.