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X-CLARITY™ Systems & Reagents for Tissue Clearing

Logos Biosystems

The X-CLARITY™ is a collection of systems and ready-to-use reagents developed by Logos Biosystems to make electrophoretic tissue clearing simple, rapid, and efficient.

Tissue clearing in 3 simple steps

1. Hydrogel Infusion

2. TISSUE-Hydrogel Hybridization

3. Lipid Removal

1. TISSUE-HYDROGEL HYBRIDIZATION

X-CLARITY™ Hydrogel Solution Kit C1310X
A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample. Once the solution has permeated the tissue, the sample is placed in the X-CLARITY™ Polymerization System. Monomers polymerize in the anaerobic environment, covalently linking the biomolecules to a sturdy hydrogel network, preserving molecular information and structural integrity.

X-CLARITY™ Polymerization System C20001
The X-CLARITY™ Polymerization System is a standalone, automated system developed to simplify tissue-hydrogel hybridization, a crucial step for optimal tissue clearing. Multiple samples can be placed in multi-well plates or conical tubes for rapid and efficient high-throughput sample processing. Users can control polymerization by adjusting vacuum strength, temperature, and a timer through a simple touchscreen interface. A ready-to-use X-CLARITY™ Hydrogel Solution Kit simplifies the process even further.

2.TISSUE CLEARING

X-CLARITY™ Tissue Clearing System II C30001
The X-CLARITY™ Tissue Clearing System II is an all-in-one, easy-to-use solution for electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues while preserving the structural integrity of the sample. Clear a whole mouse brain in just 6 hours, an astounding 8 times faster than the original technology. Electrophoretic Tissue Clearing Solution is a premixed SDS-based buffer optimized for use with the system. This advanced system ensures that tissue clearing occurs efficiently and rapidly.

3.ANTIBODY LABELING

DeepLabel™ Antibody Staining Kit C33001
The DeepLabel™ Antibody Staining Kit enhances antibody penetration into large clarified tissues for vibrant fluorescence 3D imaging. DeepLabel facilitates the diffusion of molecular probes deep into thick, protein-dense tissues for robust and efficient antibody labeling. DeepLabel has been optimized for the antibody labeling of clarified tissues.

  • Efficient antibody penetration
  • Site-specific labeling
  • Deep permeation into thick tissues
  • Simple protocol with ready-to-use reagents
  • Vibrant imaging at subcellular resolution

4. IMAGING

X-CLARITY™ Mounting Solution C13101 C13102 C13107
X-CLARITY™ Mounting Solution is a high quality, cost-efficient refractive index matching solution (RIMS) modified from the recipe developed by Yang et al. The refractive index (RI) of the solution is 1.460 at 25°C and is stable over a wide temperature range. The X-CLARITY™ Mounting Solution minimizes photobleaching and preserves fluorescence signals, making it an ideal solution for clarified and labeled tissue samples.

· RI = 1.460 at 25°C
· Minimizes photobleaching
· Preserves fluorescence signals